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flag-tagged ar plasmids with lbd mutations  (Addgene inc)


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    Addgene inc flag-tagged ar plasmids with lbd mutations
    ATC-324 degrades <t>AR-LBD</t> mutants and AR-v7/AR-FL heterodimer. A, Immunoblot analysis of AR with or without LBD mutation (L702H, F877L, T878A, M896V, and H874Y) in PC3 cells transfected with the indicated plasmid and treated with ATC-324 (5 µmol/L, 24 hours). The levels of β-actin were examined as a loading control. B and C, Immunoblot analysis of AR-FL and AR-v7 in 22Rv1 cells treated with ATC-324 at the indicated concentrations for 24 hours ( B ) and densitometry of B ( C ). The protein levels were normalized with β-actin. D and E, Fluorescence images of the PLA of FLAG and p62 in PC3 cells ( D ) and quantification of the number of red puncta ( E ). The cells were transfected with untagged AR-FL and <t>FLAG-AR-v7</t> <t>plasmids</t> and treated with indicated chemicals (5 µmol/L, 12 hours). n >30 cells. F, Immunoblot analysis of FLAG in PC3 cells transfected with indicated plasmids and treated with or without ATC-324 (5 µmol/L, 24 hours). The protein levels were normalized to β-actin and the densitometry values of FLAG corresponding to AR-FL and AR-v7 were labeled below the panels. G, Immunoblot analysis of FLAG in PC3 cells transfected with indicated plasmids and treated with or without ATC-324 (5 µmol/L, 24 hours). The protein levels were normalized to β-actin and the densitometry values of FLAG corresponding to AR-FL and AR-v7 were labeled below the panels. Data are shown as means ± SEM ( n = 3 independent experiments), and statistical significance was analyzed using one-way ANOVA, followed by Tukey post hoc procedures.
    Flag Tagged Ar Plasmids With Lbd Mutations, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/flag-tagged+ar+plasmids+with+lbd+mutations/flag+tagged+ar+plasmids+with+lbd+mutations/pmc11733533-122-4-11
    Average 90 stars, based on 1 article reviews
    flag-tagged ar plasmids with lbd mutations - by Bioz Stars, 2026-09
    90/100 stars

    Images

    1) Product Images from "An Autophagy-Targeting Chimera Induces Degradation of Androgen Receptor Mutants and AR-v7 in Castration-Resistant Prostate Cancer"

    Article Title: An Autophagy-Targeting Chimera Induces Degradation of Androgen Receptor Mutants and AR-v7 in Castration-Resistant Prostate Cancer

    Journal: Cancer Research

    doi: 10.1158/0008-5472.CAN-24-0591

    ATC-324 degrades AR-LBD mutants and AR-v7/AR-FL heterodimer. A, Immunoblot analysis of AR with or without LBD mutation (L702H, F877L, T878A, M896V, and H874Y) in PC3 cells transfected with the indicated plasmid and treated with ATC-324 (5 µmol/L, 24 hours). The levels of β-actin were examined as a loading control. B and C, Immunoblot analysis of AR-FL and AR-v7 in 22Rv1 cells treated with ATC-324 at the indicated concentrations for 24 hours ( B ) and densitometry of B ( C ). The protein levels were normalized with β-actin. D and E, Fluorescence images of the PLA of FLAG and p62 in PC3 cells ( D ) and quantification of the number of red puncta ( E ). The cells were transfected with untagged AR-FL and FLAG-AR-v7 plasmids and treated with indicated chemicals (5 µmol/L, 12 hours). n >30 cells. F, Immunoblot analysis of FLAG in PC3 cells transfected with indicated plasmids and treated with or without ATC-324 (5 µmol/L, 24 hours). The protein levels were normalized to β-actin and the densitometry values of FLAG corresponding to AR-FL and AR-v7 were labeled below the panels. G, Immunoblot analysis of FLAG in PC3 cells transfected with indicated plasmids and treated with or without ATC-324 (5 µmol/L, 24 hours). The protein levels were normalized to β-actin and the densitometry values of FLAG corresponding to AR-FL and AR-v7 were labeled below the panels. Data are shown as means ± SEM ( n = 3 independent experiments), and statistical significance was analyzed using one-way ANOVA, followed by Tukey post hoc procedures.
    Figure Legend Snippet: ATC-324 degrades AR-LBD mutants and AR-v7/AR-FL heterodimer. A, Immunoblot analysis of AR with or without LBD mutation (L702H, F877L, T878A, M896V, and H874Y) in PC3 cells transfected with the indicated plasmid and treated with ATC-324 (5 µmol/L, 24 hours). The levels of β-actin were examined as a loading control. B and C, Immunoblot analysis of AR-FL and AR-v7 in 22Rv1 cells treated with ATC-324 at the indicated concentrations for 24 hours ( B ) and densitometry of B ( C ). The protein levels were normalized with β-actin. D and E, Fluorescence images of the PLA of FLAG and p62 in PC3 cells ( D ) and quantification of the number of red puncta ( E ). The cells were transfected with untagged AR-FL and FLAG-AR-v7 plasmids and treated with indicated chemicals (5 µmol/L, 12 hours). n >30 cells. F, Immunoblot analysis of FLAG in PC3 cells transfected with indicated plasmids and treated with or without ATC-324 (5 µmol/L, 24 hours). The protein levels were normalized to β-actin and the densitometry values of FLAG corresponding to AR-FL and AR-v7 were labeled below the panels. G, Immunoblot analysis of FLAG in PC3 cells transfected with indicated plasmids and treated with or without ATC-324 (5 µmol/L, 24 hours). The protein levels were normalized to β-actin and the densitometry values of FLAG corresponding to AR-FL and AR-v7 were labeled below the panels. Data are shown as means ± SEM ( n = 3 independent experiments), and statistical significance was analyzed using one-way ANOVA, followed by Tukey post hoc procedures.

    Techniques Used: Western Blot, Mutagenesis, Transfection, Plasmid Preparation, Control, Fluorescence, Labeling

    Related Articles

    Construct:

    Article Title: An Autophagy-Targeting Chimera Induces Degradation of Androgen Receptor Mutants and AR-v7 in Castration-Resistant Prostate Cancer
    Article Snippet: pGL4.5-Luc2 plasmid was purchased form Promega (E131A). pEGFP-C1-AR (RRID: Addgene_28235) and pEGFP-C1-AR v7 (RRID: Addgene_86856) were gifts from Marco Marcelli and Michael Mancini (Baylor College of Medicine). .. Flag-tagged AR plasmids with LBD mutations were constructed utilizing Flag-M4-AR (RRID: Addgene plasmid_171240), a gift from Steven Balk (Harvard Medical School), via site-directed mutagenesis. ..

    Plasmid Preparation:

    Article Title: An Autophagy-Targeting Chimera Induces Degradation of Androgen Receptor Mutants and AR-v7 in Castration-Resistant Prostate Cancer
    Article Snippet: pGL4.5-Luc2 plasmid was purchased form Promega (E131A). pEGFP-C1-AR (RRID: Addgene_28235) and pEGFP-C1-AR v7 (RRID: Addgene_86856) were gifts from Marco Marcelli and Michael Mancini (Baylor College of Medicine). .. Flag-tagged AR plasmids with LBD mutations were constructed utilizing Flag-M4-AR (RRID: Addgene plasmid_171240), a gift from Steven Balk (Harvard Medical School), via site-directed mutagenesis. ..

    Mutagenesis:

    Article Title: An Autophagy-Targeting Chimera Induces Degradation of Androgen Receptor Mutants and AR-v7 in Castration-Resistant Prostate Cancer
    Article Snippet: pGL4.5-Luc2 plasmid was purchased form Promega (E131A). pEGFP-C1-AR (RRID: Addgene_28235) and pEGFP-C1-AR v7 (RRID: Addgene_86856) were gifts from Marco Marcelli and Michael Mancini (Baylor College of Medicine). .. Flag-tagged AR plasmids with LBD mutations were constructed utilizing Flag-M4-AR (RRID: Addgene plasmid_171240), a gift from Steven Balk (Harvard Medical School), via site-directed mutagenesis. ..

    other:

    Article Title: An Autophagy-Targeting Chimera Induces Degradation of Androgen Receptor Mutants and AR-v7 in Castration-Resistant Prostate Cancer
    Article Snippet: 2 Genetic alterations play a pivotal role in various human diseases, particularly cancer.. The androgen 39 receptor (AR) is a crucial transcription factor driving prostate cancer (PCa) progression across all 40 stages.. Current AR-targeting therapies utilize competitive AR antagonists or pathway suppressors.



    Similar Products

    flag-tagged ar plasmids with lbd mutations  (Addgene inc)
    90
    Addgene inc flag-tagged ar plasmids with lbd mutations
    ATC-324 degrades <t>AR-LBD</t> mutants and AR-v7/AR-FL heterodimer. A, Immunoblot analysis of AR with or without LBD mutation (L702H, F877L, T878A, M896V, and H874Y) in PC3 cells transfected with the indicated plasmid and treated with ATC-324 (5 µmol/L, 24 hours). The levels of β-actin were examined as a loading control. B and C, Immunoblot analysis of AR-FL and AR-v7 in 22Rv1 cells treated with ATC-324 at the indicated concentrations for 24 hours ( B ) and densitometry of B ( C ). The protein levels were normalized with β-actin. D and E, Fluorescence images of the PLA of FLAG and p62 in PC3 cells ( D ) and quantification of the number of red puncta ( E ). The cells were transfected with untagged AR-FL and <t>FLAG-AR-v7</t> <t>plasmids</t> and treated with indicated chemicals (5 µmol/L, 12 hours). n >30 cells. F, Immunoblot analysis of FLAG in PC3 cells transfected with indicated plasmids and treated with or without ATC-324 (5 µmol/L, 24 hours). The protein levels were normalized to β-actin and the densitometry values of FLAG corresponding to AR-FL and AR-v7 were labeled below the panels. G, Immunoblot analysis of FLAG in PC3 cells transfected with indicated plasmids and treated with or without ATC-324 (5 µmol/L, 24 hours). The protein levels were normalized to β-actin and the densitometry values of FLAG corresponding to AR-FL and AR-v7 were labeled below the panels. Data are shown as means ± SEM ( n = 3 independent experiments), and statistical significance was analyzed using one-way ANOVA, followed by Tukey post hoc procedures.
    Flag Tagged Ar Plasmids With Lbd Mutations, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/flag-tagged+ar+plasmids+with+lbd+mutations/flag+tagged+ar+plasmids+with+lbd+mutations/pmc11733533-122-4-11
    Average 90 stars, based on 1 article reviews
    flag-tagged ar plasmids with lbd mutations - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    ATC-324 degrades AR-LBD mutants and AR-v7/AR-FL heterodimer. A, Immunoblot analysis of AR with or without LBD mutation (L702H, F877L, T878A, M896V, and H874Y) in PC3 cells transfected with the indicated plasmid and treated with ATC-324 (5 µmol/L, 24 hours). The levels of β-actin were examined as a loading control. B and C, Immunoblot analysis of AR-FL and AR-v7 in 22Rv1 cells treated with ATC-324 at the indicated concentrations for 24 hours ( B ) and densitometry of B ( C ). The protein levels were normalized with β-actin. D and E, Fluorescence images of the PLA of FLAG and p62 in PC3 cells ( D ) and quantification of the number of red puncta ( E ). The cells were transfected with untagged AR-FL and FLAG-AR-v7 plasmids and treated with indicated chemicals (5 µmol/L, 12 hours). n >30 cells. F, Immunoblot analysis of FLAG in PC3 cells transfected with indicated plasmids and treated with or without ATC-324 (5 µmol/L, 24 hours). The protein levels were normalized to β-actin and the densitometry values of FLAG corresponding to AR-FL and AR-v7 were labeled below the panels. G, Immunoblot analysis of FLAG in PC3 cells transfected with indicated plasmids and treated with or without ATC-324 (5 µmol/L, 24 hours). The protein levels were normalized to β-actin and the densitometry values of FLAG corresponding to AR-FL and AR-v7 were labeled below the panels. Data are shown as means ± SEM ( n = 3 independent experiments), and statistical significance was analyzed using one-way ANOVA, followed by Tukey post hoc procedures.

    Journal: Cancer Research

    Article Title: An Autophagy-Targeting Chimera Induces Degradation of Androgen Receptor Mutants and AR-v7 in Castration-Resistant Prostate Cancer

    doi: 10.1158/0008-5472.CAN-24-0591

    Figure Lengend Snippet: ATC-324 degrades AR-LBD mutants and AR-v7/AR-FL heterodimer. A, Immunoblot analysis of AR with or without LBD mutation (L702H, F877L, T878A, M896V, and H874Y) in PC3 cells transfected with the indicated plasmid and treated with ATC-324 (5 µmol/L, 24 hours). The levels of β-actin were examined as a loading control. B and C, Immunoblot analysis of AR-FL and AR-v7 in 22Rv1 cells treated with ATC-324 at the indicated concentrations for 24 hours ( B ) and densitometry of B ( C ). The protein levels were normalized with β-actin. D and E, Fluorescence images of the PLA of FLAG and p62 in PC3 cells ( D ) and quantification of the number of red puncta ( E ). The cells were transfected with untagged AR-FL and FLAG-AR-v7 plasmids and treated with indicated chemicals (5 µmol/L, 12 hours). n >30 cells. F, Immunoblot analysis of FLAG in PC3 cells transfected with indicated plasmids and treated with or without ATC-324 (5 µmol/L, 24 hours). The protein levels were normalized to β-actin and the densitometry values of FLAG corresponding to AR-FL and AR-v7 were labeled below the panels. G, Immunoblot analysis of FLAG in PC3 cells transfected with indicated plasmids and treated with or without ATC-324 (5 µmol/L, 24 hours). The protein levels were normalized to β-actin and the densitometry values of FLAG corresponding to AR-FL and AR-v7 were labeled below the panels. Data are shown as means ± SEM ( n = 3 independent experiments), and statistical significance was analyzed using one-way ANOVA, followed by Tukey post hoc procedures.

    Article Snippet: Flag-tagged AR plasmids with LBD mutations were constructed utilizing Flag-M4-AR (RRID: Addgene plasmid_171240), a gift from Steven Balk (Harvard Medical School), via site-directed mutagenesis.

    Techniques: Western Blot, Mutagenesis, Transfection, Plasmid Preparation, Control, Fluorescence, Labeling